|
KMID : 1134820210500111258
|
|
Journal of the Korean Society of Food Science and Nutrition
2021 Volume.50 No. 11 p.1258 ~ p.1263
|
|
|
Validation of Analytical Method and Procyanidin B2, C1 Content in Korean Apple Cultivars
|
|
Kim Ji-Yeong
Jang Ha-na
Kim Gi-Chang
Kang Hae-Ju
Hwang In-Guk
|
|
|
Abstract
|
|
|
|
Procyanidin is a major functional component of apples. This study sought to verify an analytical method to evaluate the procyanidin B2 and C1 content by varieties, parts, and farms of apples. The linearity of the method developed using ultra performance liquid chromatography (UPLC) was excellent, and the correlation coefficients (R2) of procyanidin B2 and C1 were 0.9996 and 0.9996, respectively. The limits of detection of procyanidin B2 and C1 were 0.70 and 0.77 μg/mL and limits of quantification were 2.14 and 2.32 μg/mL, respectively. The relative standard deviation (RSD) of the intra-day and inter-day were calculated and had excellent precision and was found to be within 5%. The RSD of the recovery rate of procyanidin B2 and C1 were 0.68∼3.33% and 1.63∼2.78%, respectively. Through this, the analytical method for estimating procyanidin was confirmed. Analysis of procyanidin in the different apple varieties (Summerking, Gamhong, Hongro, Arisu, Busa), showed that Gamhong had significantly (P<0.05) higher procyanidin B2 and C1 content compared to other varieties, and the peel had the highest procyanidin B2 and C1 content. When the procyanidin content of Gamhong collected from ten farms was analyzed, the average content of procyanidin B2 in the peel was 9.79 mg/100 g FW and that of procyanidin C1 was 11.58 mg/100 g FW. Heretofore, information on the procyanidin content in apples grown in Korea was insufficient. Therefore, it is considered that the results of this study can be considered necessary to improve the usability of apples.
|
|
|
KEYWORD
|
|
|
apple, procyanidin B2, procyanidin C1, UPLC, analytical method
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|